Inbred maize line phajo

ABSTRACT

An inbred maize line, designated PHAJ0, the plants and seeds of inbred maize line PHAJ0, methods for producing a maize plant produced by crossing the inbred line PHAJ0 with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PHAJ0 with another maize line or plant.

FIELD OF THE INVENTION

This invention is in the field of maize breeding, specifically relating to an inbred maize line designated PHAJ0.

BACKGROUND OF THE INVENTION

The goal of plant breeding is to combine in a single variety or hybrid various desirable traits. For field crops, these traits may include resistance to diseases and insects, tolerance to heat and drought, reducing the time to crop maturity, greater yield, and better agronomic quality. With mechanical harvesting of many crops, uniformity of plant characteristics such as germination and stand establishment, growth rate, maturity, and plant and ear height, is important.

Field crops are bred through techniques that take advantage of the plant's method of pollination. A plant is self-pollinated if pollen from one flower is transferred to the same or another flower of the same plant. A plant is cross-pollinated if the pollen comes from a flower on a different plant.

Plants that have been self-pollinated and selected for type for many generations become homozygous at almost all gene loci and produce a uniform population of true breeding progeny. A cross between two different homozygous lines produces a uniform population of hybrid plants that may be heterozygous for many gene loci. A cross of two plants each heterozygous at a number of gene loci will produce a population of hybrid plants that differ genetically and will not be uniform.

Maize (zea mays L.), often referred to as corn in the United States, can be bred by both self-pollination and cross-pollination techniques. Maize has separate male and female flowers on the same plant, located on the tassel and the ear, respectively. Natural pollination occurs in maize when wind blows pollen from the tassels to the silks that protrude from the tops of the ears.

A reliable method of controlling male fertility in plants offers the opportunity for improved plant breeding. This is especially true for development of maize hybrids, which relies upon some sort of male sterility system. There are several options for controlling male fertility available to breeders, such as: manual or mechanical emasculation (or detasseling), cytoplasmic male sterility, genetic male sterility, gametocides and the like.

Hybrid maize seed is typically produced by a male sterility system incorporating manual or mechanical detasseling. Alternate strips of two inbred varieties of maize are planted in a field, and the pollen-bearing tassels are removed from one of the inbreds (female). Providing that there is sufficient isolation from sources of foreign maize pollen, the ears of the detasseled inbred will be fertilized only from the other inbred (male), and the resulting seed is therefore hybrid and will form hybrid plants.

The laborious, and occasionally unreliable, detasseling process can be avoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMS inbred are male sterile as a result of factors resulting from the cytoplasmic, as opposed to the nuclear, genome. Thus, this characteristic is inherited exclusively through the female parent in maize plants, since only the female provides cytoplasm to the fertilized seed. CMS plants are fertilized with pollen from another inbred that is not male-sterile. Pollen from the second inbred may or may not contribute genes that make the hybrid plants male-fertile. Usually seed from detasseled fertile maize and CMS produced seed of the same hybrid are blended to insure that adequate pollen loads are available for fertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterility available, such as multiple mutant genes at separate locations within the genome that confer male sterility, as disclosed in U.S. Pat. Nos. 4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations as described by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. These and all patents referred to are incorporated by reference. In addition to these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. patent application Ser. No. 07/848,433, have developed a system of nuclear male sterility which includes: identifying a gene which is critical to male fertility; silencing this native gene which is critical to male fertility; removing the native promoter from the essential male fertility gene and replacing it with an inducible promoter; inserting this genetically engineered gene back into the plant; and thus creating a plant that is male sterile because the inducible promoter is not "on" resulting in the male fertility gene not being transcribed. Fertility is restored by inducing, or turning "on", the promoter, which in turn allows the gene that confers male fertility to be transcribed.

There are many other methods of conferring genetic male sterility in the art, each with its own benefits and drawbacks. These methods use a variety of approaches such as delivering into the plant a gene encoding a cytotoxic substance associated with a male tissue specific promoter or an antisense system in which a gene critical to fertility is identified and an antisense to that gene is inserted in the plant (see: Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCT application PCT/CA90/00037 published as WO 90/08828).

Another system useful in controlling male sterility makes use of gametocides. Gametocides are not a genetic system, but rather a topical application of chemicals. These chemicals affect cells that are critical to male fertility. The application of these chemicals affects fertility in the plants only for the growing season in which the gametocide is applied (see Carlson, Glenn R., U.S. Pat. No. 4,936,904). Application of the gametocide, timing of the application and genotype specificity often limit the usefulness of the approach.

The use of male sterile inbreds is but one factor in the production of maize hybrids. The development of maize hybrids requires, in general, the development of homozygous inbred lines, the crossing of these lines, and the evaluation of the crosses. Pedigree breeding and recurrent selection breeding methods are used to develop inbred lines from breeding populations. Breeding programs combine the genetic backgrounds from two or more inbred lines or various other broad-based sources into breeding pools from which new inbred lines are developed by selfing and selection of desired phenotypes. The new inbreds are crossed with other inbred lines and the hybrids from these crosses are evaluated to determine which of those have commercial potential.

Pedigree breeding starts with the crossing of two genotypes, each of which may have one or more desirable characteristics that is lacking in the other or which complements the other. If the two original parents do not provide all the desired characteristics, other sources can be included in the breeding population. In the pedigree method, superior plants are selfed and selected in successive generations. In the succeeding generations the heterozygous condition gives way to homogeneous lines as a result of self-pollination and selection. Typically in the pedigree method of breeding five or more generations of selfing and selection is practiced: F₁ →F₂ ; F₃ →F₄ ; F₄ →F₅, etc.

Recurrent selection breeding, backcrossing for example, can be used to improve an inbred line. Backcrossing can be used to transfer a specific desirable trait from one inbred or source to an inbred that lacks that trait. This can be accomplished, for example, by first crossing a superior inbred (recurrent parent) to a donor inbred (non-recurrent parent), that carries the appropriate gene(s) for the trait in question. The progeny of this cross is then mated back to the superior recurrent parent followed by selection in the resultant progeny for the desired trait to be transferred from the non-recurrent parent. After five or more backcross generations with selection for the desired trait, the progeny will be heterozygous for loci controlling the characteristic being transferred, but will be like the superior parent for most or almost all other genes. The last backcross generation is then selfed to give pure breeding progeny for the gene(s) being transferred.

A single cross hybrid maize variety is the cross of two inbred lines, each of which has a genotype that complements the genotype of the other. The hybrid progeny of the first generation is designated F₁. In the development of hybrids only the F₁ hybrid plants are sought. Preferred F₁ hybrids are more vigorous than their inbred parents. This hybrid vigor, or heterosis, can be manifested in many polygenic traits, including increased vegetative growth and increased yield.

The development of a hybrid maize variety involves three steps: (1) the selection of plants from various germplasm pools for initial breeding crosses; (2) the selfing of the selected plants from the breeding crosses for several generations to produce a series of inbred lines, which, although different from each other, breed true and are highly uniform; and (3) crossing the selected inbred lines with different inbred lines to produce the hybrid progeny (F₁). During the inbreeding process in maize, the vigor of the lines decreases. Vigor is restored when two different inbred lines are crossed to produce the hybrid progeny (F₁). An important consequence of the homozygosity and homogeneity of the inbred lines is that the hybrid between any two inbreds will always be the same. Once the inbreds that give a superior hybrid have been identified, the hybrid seed can be reproduced indefinitely as long as the homogeneity of the inbred parents is maintained.

A single cross hybrid is produced when two inbred lines are crossed to produce the F₁ progeny. A double cross hybrid is produced from four inbred lines crossed in pairs (A×B and C×D) and then the two F₁ hybrids are crossed again (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁ hybrids is lost in the next generation (F₂). Consequently, seed from hybrid varieties is not used for planting stock.

Maize is an important and valuable field crop. Thus, a continuing goal of plant breeders is to develop high-yielding maize hybrids that are agronomically sound based on stable inbred lines. The reasons for this goal are obvious: to maximize the amount of grain produced with the inputs used and minimize susceptibility of the crop to environmental stresses. To accomplish this goal, the maize breeder must select and develop superior inbred parental lines for producing hybrids. This requires identification and selection of genetically unique individuals that occur in a segregating population. The segregating population is the result of a combination of crossover events plus the independent assortment of specific combinations of alleles at many gene loci that results in specific genotypes. Based on the number of segregating genes, the frequency of occurrence of an individual with a specific genotype is less than 1 in 10,000. Thus, even if the entire genotype of the parents has been characterized and the desired genotype is known, only a few if any individuals having the desired genotype may be found in a large F₂ or S₀ population. Typically, however, the genotype of neither the parents nor the desired genotype is known in any detail.

In addition to the preceding problem, it is not known how the genotype will react with the environment. This genotype by environment interaction is an important, yet unpredictable, factor in plant breeding. A breeder of ordinary skill in the art cannot predict the genotype, how that genotype will interact with various environments or the resulting phenotypes of the developing lines, except perhaps in a very broad and general fashion. A breeder of ordinary skill in the art would also be unable to recreate the same line twice from the very same original parents as the breeder is unable to direct how the genomes combine or how they will interact with the environmental conditions. This unpredictability results in the expenditure of large amounts of research resources in the development of a superior new maize inbred line.

Pioneer research station staff propose about 400 to 500 new inbreds each year from over 2,000,000 pollinations. Of those proposed new inbreds, less than 50 and more commonly less than 30 are actually selected for commercial use.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred maize line, designated PHAJ0. This invention thus relates to the seeds of inbred maize line PHAJ0, to the plants of inbred maize line PHAJ0, and to methods for producing a maize plant produced by crossing the inbred line PHAJ0 with itself or another maize line. This invention further relates to hybrid maize seeds and plants produced by crossing the inbred line PHAJ0 with another maize line.

Definitions

In the description and examples that follow, a number of terms are used herein. In order to provide a clear and consistent understanding of the specification and claims, including the scope to be given such terms, the following definitions are provided. NOTE: ABS is in absolute terms and % MN is percent of the mean for the experiments in which the inbred or hybrid was grown. These designators will follow the descriptors to denote how the values are to be interpreted. Below are the descriptors used in the data tables included herein.

ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9 visual rating indicating the resistance to Anthracnose Stalk Rot. A higher score indicates a higher resistance.

BAR PLT=BARREN PLANTS. The percent of plants per plot that were not barren (lack ears).

BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near the time of pollination, and is an indication of whether a hybrid or inbred would snap or break near the time of flowering under severe winds. Data are presented as percentage of plants that did not snap.

BU ACR=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushels per acre adjusted to 15.5% moisture.

CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chlorotic mottle virus (MCMV) in combination with either maize dwarf mosaic virus (MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visual rating indicating the resistance to Corn Lethal Necrosis. A higher score indicates a higher resistance.

COM RST=COMMON RUST (Puccinia sorghi). A 1 to 9 visual rating indicating the resistance to Common Rust. A higher score indicates a higher resistance.

D/D=DRYDOWN. This represents the relative rate at which a hybrid will reach acceptable harvest moisture compared to other hybrids on a 1-9 rating scale. A high score indicates a hybrid that dries relatively fast while a low score indicates a hybrid that dries slowly.

DIP ERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Dipiodia macrospora). A 1 to 9 visual rating indicating the resistance to Diplodia Ear Mold. A higher score indicates a higher resistance.

DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plot and represents the percentage of plants that did not drop ears prior to harvest.

D/T=DROUGHT TOLERANCE. This represents a 1-9 rating for drought tolerance, and is based on data obtained under stress conditions. A high score indicates good drought tolerance and a low score indicates poor drought tolerance.

EAR HT=EAR HEIGHT. The ear height is a measure from the ground to the highest placed developed ear node attachment and is measured in inches.

EAR MLD=General Ear Mold. Visual rating (1-9 score) where a "1" is very susceptible and a "9" is very resistant. This is based on overall rating for ear mold of mature ears without determining the specific mold organism, and may not be predictive for a specific ear mold.

EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher the rating the larger the ear size.

ECB 1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinia nubilalis). A 1 to 9 visual rating indicating the resistance to preflowering leaf feeding by first generation European Corn Borer. A higher score indicates a higher resistance.

ECB 2It=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING (Ostrinia nubilalis). Average inches of tunneling per plant in the stalk.

ECB 2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostrinia nubilalis). A 1 to 9 visual rating indicating post flowering degree of stalk breakage and other evidence of feeding by European Corn Borer, Second Generation. A higher score indicates a higher resistance.

ECB DPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Dropped ears due to European Corn Borer. Percentage of plants that did not drop ears under second generation corn borer infestation.

EST CNT=EARLY STAND COUNT. This is a measure of the stand establishment in the spring and represents the number of plants that emerge on per plot basis for the inbred or hybrid.

EYE SPT=Eye Spot (Kabatiella zeae or Aureobasidium zeae). A 1 to 9 visual rating indicating the resistance to Eye Spot. A higher score indicates a higher resistance.

FUS ERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforme or Fusarium subglutinans). A 1 to 9 visual rating indicating the resistance to Fusarium ear rot. A higher score indicates a higher resistance.

GDU=Growing Degree Units. Using the Barger Heat Unit Theory, which assumes that maize growth occurs in the temperature range 50° F.-86° F. and that temperatures outside this range slow down growth; the maximum daily heat unit accumulation is 36 and the minimum daily heat unit accumulation is 0. The seasonal accumulation of GDU is a major factor in determining maturity zones.

GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heat units required for an inbred line or hybrid to have approximately 50 percent of the plants shedding pollen and is measured from the time of planting. Growing degree units are calculated by the Barger Method, where the heat units for a 24-hour period are: ##EQU1##

The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDUs to reach various stages of plant development.

GDU SLK=GDU TO SILK. The number of growing degree units required for an inbred line or hybrid to have approximately 50 percent of the plants with silk emergence from time of planting. Growing degree units are calculated by the Barger Method as given in GDU SHD definition.

GIB ERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9 visual rating indicating the resistance to Gibberella Ear Rot. A higher score indicates a higher resistance.

GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis). A 1 to 9 visual rating indicating the resistance to Gray Leaf Spot. A higher score indicates a higher resistance.

GOS WLT=Goss' Wilt (Corynebacterium nebraskense). A 1 to 9 visual rating indicating the resistance to Goss' Wilt. A higher score indicates a higher resistance.

GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the general appearance of the shelled grain as it is harvested based on such factors as the color of harvested grain, any mold on the grain, and any cracked grain. High scores indicate good grain quality.

H/POP=YIELD AT HIGH DENSITY. Yield ability at relatively high plant densities on 1-9 relative rating system with a higher number indicating the hybrid responds well to high plant densities for yield relative to other hybrids. A 1, 5, and 9 would represent very poor, average, and very good yield response, respectively, to increased plant density.

HC BLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporium carbonum). A 1 to 9 visual rating indicating the resistance to Helminthosporium infection. A higher score indicates a higher resistance.

HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates the percentage of plants not infected.

INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acre assuming drying costs of two cents per point above 15.5 percent harvest moisture and current market price per bushel.

INCOME/ACRE. Income advantage of hybrid to be patented over other hybrid on per acre basis.

INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1 over variety #2.

L/POP=YIELD AT LOW DENSITY. Yield ability at relatively low plant densities on a 1-9 relative system with a higher number indicating the hybrid responds well to low plant densities for yield relative to other hybrids. A 1, 5, and 9 would represent very poor, average, and very good yield response, respectively, to low plant density.

MDM CPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus and MCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating the resistance to Maize Dwarf Mosaic Complex. A higher score indicates a higher resistance.

MST=HARVEST MOISTURE. The moisture is the actual percentage moisture of the grain at harvest.

MST ADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 over variety #2 as calculated by: MOISTURE of variety #2-MOISTURE of variety #1=MOISTURE ADVANTAGE of variety #1.

NLF BLT=Northern Leaf Blight (Helminthosporium turcicum or Exserohilum turcicum). A 1 to 9 visual rating indicating the resistance to Northern Leaf Blight. A higher score indicates a higher resistance.

PLT HT=PLANT HEIGHT. This is a measure of the height of the plant from the ground to the tip of the tassel in inches.

POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount of pollen shed. The higher the score the more pollen shed.

POL WT=POLLEN WEIGHT. This is calculated by dry weight of tassels collected as shedding commences minus dry weight from similar tassels harvested after shedding is complete.

It should be understood that the inbred can, through routine manipulation of cytoplasmic or other factors, be produced in a male-sterile form. Such embodiments are also contemplated within the scope of the present claims.

POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.

POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage of variety #1 over variety #2 as calculated by PLANT POPULATION of variety #2-PLANT POPULATION of variety #1=PLANT POPULATION ADVANTAGE of variety #1.

PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relative maturity, is based on the harvest moisture of the grain. The relative maturity rating is based on a known set of checks and utilizes standard linear regression analyses and is also referred to as the Comparative Relative Maturity Rating System that is similar to the Minnesota Relative Maturity Rating System.

PRM SHD=A relative measure of the growing degree units (GDU) required to reach 50% pollen shed. Relative values are predicted values from the linear regression of observed GDU's on relative maturity of commercial checks.

RT LDG=ROOT LODGING. Root lodging is the percentage of plants that do not root lodge; plants that lean from the vertical axis at an approximately 30° angle or greater would be counted as root lodged.

RTL ADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1 over variety #2.

SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount of scatter grain (lack of pollination or kernel abortion) on the ear. The higher the score the less scatter grain.

SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amount of vegetative growth after emergence at the seedling stage (approximately five leaves). A higher score indicates better vigor.

SEL IND=SELECTION INDEX. The selection index gives a single measure of the hybrid's worth based on information for up to five traits. A maize breeder may utilize his or her own set of traits for the selection index. One of the traits that is almost always included is yield. The selection index data presented in the tables represent the mean value averaged across testing stations.

SLF BLT=SOUTHERN LEAF BLIGHT (Helminthosporium maydis or Bipolaris maydis). A 1 to 9 visual rating indicating the resistance to Southern Leaf Blight. A higher score indicates a higher resistance.

SOU RST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual rating indicating the resistance to Southern Rust. A higher score indicates a higher resistance.

STA GRN=STAY GREEN. Stay green is the measure of plant health near the time of black layer formation (physiological maturity). A high score indicates better late-season plant health.

STD ADV=STALK STANDING ADVANTAGE. The advantage of variety #1 over variety #2 for the trait STK CNT.

STK CNT=NUMBER OF PLANTS. This is the final stand or number of plants per plot.

STK LDG=STALK LODGING. This is the percentage of plants that did not stalk lodge (stalk breakage) as measured by either natural lodging or pushing the stalks and determining the percentage of plants that break below the ear.

STW WLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual rating indicating the resistance to Stewart's Wilt. A higher score indicates a higher resistance.

TAS BLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure the degree of blasting (necrosis due to heat stress) of the tassel at the time of flowering. A 1 would indicate a very high level of blasting at time of flowering, while a 9 would have no tassel blasting.

TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate the relative size of the tassel. The higher the rating the larger the tassel.

TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams) just prior to pollen shed.

TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate the relative hardness (smoothness of crown) of mature grain. A 1 would be very soft (extreme dent) while a 9 would be very hard (flinty or very smooth crown).

TILLER=TILLERS. A count of the number of tillers per plot that could possibly shed pollen was taken. Data is given as a percentage of tillers: number of tillers per plot divided by number of plants per plot.

TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grain in pounds for a given volume (bushel).

TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain in pounds for a given volume (bushel) adjusted for 15.5 percent moisture.

TSW ADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1 over variety #2.

WIN M %=PERCENT MOISTURE WINS.

WIN Y %=PERCENT YIELD WINS.

YLD=YIELD. It is the same as BU ACR ABS.

YLD ADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety #2 as calculated by: YIELD of variety #1-YIELD variety #2=yield advantage of variety #1.

YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relative rating for yield based on plot ear piles. The higher the rating the greater visual yield appearance.

DETAILED DESCRIPTION OF THE INVENTION

Inbred maize lines are typically developed for use in the production of hybrid maize lines. Inbred maize lines need to be highly homogeneous, homozygous and reproducible to be useful as parents of commercial hybrids. There are many analytical methods available to determine the homozygotic and phenotypic stability of these inbred lines.

The oldest and most traditional method of analysis is the observation of phenotypic traits. The data is usually collected in field experiments over the life of the maize plants to be examined. Phenotypic characteristics most often observed are for traits associated with plant morphology, ear and kernel morphology, insect and disease resistance, maturity, and yield.

In addition to phenotypic observations, the genotype of a plant can also be examined. There are many laboratory-based techniques available for the analysis, comparison and characterization of plant genotype; among these are Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats (SSRs) which are also referred to as Microsatellites.

The most widely used of these laboratory techniques are Isozyme Electrophoresis and RFLPs as discussed in Lee, M., "Inbred Lines of Maize and Their Molecular Markers," The Maize Handbook, (Springer-Verlag, New York, Inc. 1994, at 423-432) incorporated herein by reference. Isozyme Electrophoresis is a useful tool in determining genetic composition, although it has relatively low number of available markers and the low number of allelic variants among maize inbreds. RFLPs have the advantage of revealing an exceptionally high degree of allelic variation in maize and the number of available markers is almost limitless.

Maize RFLP linkage maps have been rapidly constructed and widely implemented in genetic studies. One such study is described in Boppenmaier, et al., "Comparisons among strains of inbreds for RFLPs", Maize Genetics Cooperative Newsletter, 65:1991, pg. 90, is incorporated herein by reference. This study used 101 RFLP markers to analyze the patterns of 2 to 3 different deposits each of five different inbred lines. The inbred lines had been selfed from 9 to 12 times before being adopted into 2 to 3 different breeding programs. It was results from these 2 to 3 different breeding programs that supplied the different deposits for analysis. These five lines were maintained in the separate breeding programs by selfing or sibbing and rogueing off-type plants for an additional one to eight generations. After the RFLP analysis was completed, it was determined the five lines showed 0-2% residual heterozygosity. Although this was a relatively small study, it can be seen using RFLPs that the lines had been highly homozygous prior to the separate strain maintenance.

Inbred maize line PHAJ0 is a yellow, flint maize inbred that is best suited as a male in crosses for producing first generation F₁ maize hybrids. Inbred maize line PHAJ0 is best adapted to the Northeast region of the United States and can be used to produce hybrids from approximately 82 relative maturity based on the Comparative Relative Maturity Rating System for harvest moisture of grain. Inbred maize line PHAJ0 has an acceptable yield for its maturity. Inbred PHAJ0 has excellent stay green, very good early growth, solid stalk strength, good roots, good dry down, and grows well in high density plantings. Inbred PHAJ0 produces thin eared hybrids. PHAJ0 is also unique in its ability to produce plants with very dark green, healthy leaves and bright yellow anthers.

The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. The inbred has been self-pollinated and ear-rowed a sufficient number of generations with careful attention paid to uniformity of plant type to ensure the homozygosity and phenotypic stability necessary to use in commercial production. The line has been increased both by hand and in isolated fields with continued observation for uniformity. No variant traits have been observed or are expected in PHAJ0.

Inbred maize line PHAJ0, being substantially homozygous, can be reproduced by planting seeds of the line, growing the resulting maize plants under self-pollinating or sib-pollinating conditions with adequate isolation, and harvesting the resulting seed, using techniques familiar to the agricultural arts.

                                      TABLE 1                                      __________________________________________________________________________     VARIETY DESCRIPTION INFORMATION                                                INBRED = PHAJ0                                                                 Type: Dent                                                                     Region Best Adapted: Northeast                                                 __________________________________________________________________________     A.                                                                               Maturity:                 Average across maturity zones.                                                                        Zone: 0                       GDU Shed:                 1130                                                 GDU Silk:                 1160                                                 No. Reps:                 23                                                 B.                                                                               Plant Characteristics:                                                         Plant height (to tassel tip):                                                                            184 cm                                               Length of top ear internode:                                                                             14 cm                                                Number of ears per stalk: Single                                               Ear height (to base of top ear):                                                                         73 cm                                                Number of tillers:        None                                                 Cytoplasm type:           Normal                                             C.                                                                               Leaf:                                                                          Color:                    (K166) Very Dark Green*                              Angle from Stalk:         30-60 degrees                                        Marginal Waves:           (WF9) Few                                            Number of Leaves (mature plants):                                                                        15                                                   Sheath Pubescence:        (W22) Light                                          Longitudinal Creases:     (PA11) Many                                          Length (Ear node leaf):   65 cm                                                Width (widest point, ear node leaf):                                                                     8 cm                                               D.                                                                               Tassel:                                                                        Number lateral branches:  10                                                   Branch Angle from central spike:                                                                         >45 degrees                                          Pollen Shed:              Light based on Pollen Yield Test                                               (93% of experiment means)                            Peduncle Length (top leaf to basal branches):                                                            22 cm                                                Anther Color:             Yellow*                                              Glume Color:              Red*                                               E.                                                                               Ear                                                                            (Husked Ear Data Except When Stated Otherwise):                                Length:                   15 cm                                                Weight:                   85 gm                                                Mid-point Diameter:       36 mm                                                Silk Color:               Pink*                                                Husk Extension (Harvest stage):                                                                          Medium (barely covering ear)                         Husk Leaf:                Long (>15 cm)                                        Taper of Ear:             Slight                                               Position of Shank (dry husks):                                                                           Upright                                              Kernel Rows:              Straight, Distinct     Number: 14                    Husk Color (fresh):       Light Green*                                         Husk Color (dry):         Buff*                                                Shank Length:             7 cm                                                 Shank (No. of internodes):                                                                               8                                                  F.                                                                               Kernel (Dried):                                                                Size (from ear mid-point):                                                     Length:                   10 mm                                                Width:                    8 mm                                                 Thick:                    5 mm                                                 Shape Grade (% rounds):   20-40                                                                          (40% medium round based on Parent Test Data)         Pericarp Color:           Colorless*                                           Aleurone Color:           Homozygous Yellow*                                   Endosperm Color:          Yellow*                                              Endosperm Type:           Normal Starch                                        Gm Wt/100 Seeds (unsized):                                                                               26 gm                                              G.                                                                               Cob:                                                                           Diameter at mid-point:    22 mm                                                Strength:                 Weak                                                 Color:                    Red*                                               H.                                                                               Diseases:                                                                      Carbonum Leaf Blight (H. carbonum):                                                                      Intermediate                                         N. Leaf Blight (E. turcicum):                                                                            Susceptible                                          Eye Spot (K. zeae):       Susceptible                                          Goss's Wilt (C. nebraskense):                                                                            Resistant                                            Fusarium Ear Mold (F. moniliforme):                                                                      Susceptible                                          Gibberella Ear Rot (G. zeae):                                                                            Resistant                                          I.                                                                               Insects:                                                                       European Corn Borer-1 Leaf Damage (Pre-flowering):                                                       Susceptible                                          European Corn Borer-2 (Post-flowering):                                                                  Susceptible                                        The above descriptions are based on a scale of 1-9, 1 being highly             susceptible,                                                                   9 being highly resistant                                                         S (Susceptible):          Would generally represent a score of 1-3.            I (Intermediate):         Would generally represent a score of 4-5.            R (Resistant):            Would generally represent a score of 6-7.            H (Highly Resistant):     Would generally represent a score of 8-9.                                      Highly resistant does not imply the inbred is                                  immune.                                            J.                                                                               Variety Most Closely Resembling:                                               Character:                Inbred                                               Maturity                  PHK05                                                Usage                     PHK05                                              __________________________________________________________________________      *In interpreting the foregoing color designations, reference may be had t      the Munsell Glossy Book of Color, a standard color reference.                  Data for Items B, C, D, E, F, and G is based primarily on a maximum of         four reps from Johnston, Iowa grown in 1993 and 1994, plus description         information from the maintaining station.                                      PHK05 (PVP Certificate No. 8800001) is a Pioneer HiBred International,         Inc. proprietary inbred.                                                 

Industrial Applicability

This invention also is directed to methods for producing a maize plant by crossing a first parent maize plant with a second parent maize plant wherein the first or second parent maize plant is an inbred maize plant of the line PHAJ0. Further, both first and second parent maize plants can come from the inbred maize line PHAJ0. Thus, any such methods using the inbred maize line PHAJ0 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, and the like. All plants produced using inbred maize line PHAJ0 as a parent are within the scope of this invention. Advantageously, the inbred maize line is used in crosses with other, different, maize inbreds to produce first generation (F₁) maize hybrid seeds and plants with superior characteristics.

As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which maize plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, ovules, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165:322-332 reflects that 97% of the plants cultured that produced callus were capable of plant regeneration. Subsequent experiments with both inbreds and hybrids produced 91% regenerable callus that produced plants. In a further study in 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988), 7:262-265 reports several media additions that enhance regenerability of callus of two inbred lines. Other published reports also indicated that "nontraditional" tissues are capable of producing somatic embryogenesis and plant regeneration. K. P. Rao, et al., Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger, et al., Plant Cell Reports, 6:345-347 (1987) indicates somatic embryogenesis from the tissue cultures of maize leaf segments. Thus, it is clear from the literature that the state of the art is such that these methods of obtaining plants are, and were, "conventional" in the sense that they are routinely used and have a very high rate of success.

Tissue culture of maize is described in European Patent Application, publication 160,390, incorporated herein by reference. Maize tissue culture procedures are also described in Green and Rhodes, "Plant Regeneration in Tissue Culture of Maize," Maize for Biological Research (Plant Molecular Biology Association, Charlottesville, Va. 1982, at 367-372) and in Duncan, et al., "The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes," 165 Planta 322-332 (1985). Thus, another aspect of this invention is to provide cells which upon growth and differentiation produce maize plants having the physiological and morphological characteristics of inbred line PHAJ0.

Maize is used as human food, livestock feed, and as raw material in industry. The food uses of maize, in addition to human consumption of maize kernels, include both products of dry- and wet-milling industries. The principal products of maize dry milling are grits, meal and flour. The maize wet-milling industry can provide maize starch, maize syrups, and dextrose for food use. Maize oil is recovered from maize germ, which is a by-product of both dry- and wet-milling industries.

Maize, including both grain and non-grain portions of the plant, is also used extensively as livestock feed, primarily for beef cattle, dairy cattle, hogs, and poultry.

Industrial uses of maize include production of ethanol, maize starch in the wet-milling industry and maize flour in the dry-milling industry. The industrial applications of maize starch and flour are based on functional properties, such as viscosity, film formation, adhesive properties, and ability to suspend particles. The maize starch and flour have application in the paper and textile industries. Other industrial uses include applications in adhesives, building materials, foundry binders, laundry starches, explosives, oil-well muds, and other mining applications.

Plant parts other than the grain of maize are also used in industry: for example, stalks and husks are made into paper and wallboard and cobs are used for fuel and to make charcoal.

The seed of inbred maize line PHAJ0, the plant produced from the inbred seed, the hybrid maize plant produced from the crossing of the inbred, hybrid seed, and various parts of the hybrid maize plant can be utilized for human food, livestock feed, and as a raw material in industry.

Performance Examples of PHAJ0

In the examples that follow, the traits and characteristics of inbred maize line PHAJ0 are presented as an inbred per se and in hybrid combination. The data collected on inbred maize line PHAJ0 is presented for key characteristics and traits.

The inbred per se results in Table 2A compare PHAJ0 to its parent, PHK05. The results show PHAJ0 higher yielding and has a higher test weight than PHK05. Inbred PHAJ0 has a lower percent of tillers than does PHK05. Inbred PHAJ0 has higher resistance to root lodging and higher ear texture scores than PHK05.

Table 2B compares PHAJ0 to its other parent, PHJ63. The inbred per se results show inbred PHJ63 has higher yield and harvest moisture. Inbreds PHAJ0 and PHJ63 have similar test weights. Inbred PHAJ0 has early GDU to shed and GDU to silk scores than inbred PHJ63.

Table 2C compares the inbred per se results of PHAJ0 and PHW06. Inbred PHW06 demonstrates a somewhat higher bushel per acre yield than PHAJ0. Inbred PHAJ0 shows a slightly higher seedling vigor and early stand count than inbred PHW06. Inbred PHAJ0 demonstrates a lower GDU to shed and GDU to silk score than PHW06. In addition, PHAJ0 has significantly better pollen trait scores than PHW06.

Table 2D inbred per se results compare PHAJ0 to PHJ40. Inbred PHJ40 shows a higher yield than PHAJ0, but the two inbreds have similar test weights. Inbred PHAJ0 has a lower GDU to shed and GDU to silk score than inbred PHJ40.

Table 3A compares the general combining ability of PHAJ0 to PHK05. PHK05 hybrids show a higher seedling vigor than PHAJ0 hybrids, but the yields of the two are similar. PHAJ0 hybrids have a higher resistance to root lodging and a higher stay green score.

Table 4A compares the specific combining ability of PHAJ0 to PH54B. The PHAJ0 hybrid is generated by crossing PHAJ0 and PHRE1. The PH54B hybrid is generated by crossing PH54B and PHRE1. The PH54B hybrid is higher yielding than the PHAJ0 hybrid, however, the PHAJ0 hybrid has a higher test weight than the PH54B hybrid. The PHAJ0 hybrid has a significantly higher seedling vigor and stay green score. The PHAJ0 hybrid is significantly earlier as s measured by GDU to shed and GDU to silk than the PH54B hybrid.

The specific combining ability results of Table 4B compare a PHAJ0 hybrid to a PH90A hybrid. The PHAJ0 hybrid is produced by crossing inbred PHAJ0 and inbred PHRE1. The PH90A hybrid is produced by crossing inbred PH90A and inbred PHRE 1. The yield and test weight results are similar for the two hybrids. The PHAJ0 hybrid has higher seedling vigor and early stand count than the PH90A hybrid. In addition, the PHAJ0 hybrid has lower GDU to shed and GDU to silk scores. The PHAJ0 hybrid also has significantly higher stay green than the PH90A hybrid.

The specific combining ability results of Table 4C compare a PHAJ0 is hybrid to a PHKM5 hybrid. The PHAJ0 hybrid is generated by crossing PHAJ0 and PHRE 1. The PHKM5 hybrid is generated by crossing PHKM5 and PHRE 1. The PHKM5 hybrid has a higher yield than the PHAJ0 hybrid, however, the PHKM5 hybrid has a similar test weight as the PHAJ0 hybrid. The PHAJ0 hybrid has lower GDU to shed and GDU to silk scores than the PHKM5 hybrid.

Table 4D specific combining ability results compare a PHAJ0 hybrid to a PHBF0 hybrid. The PHAJ0 hybrid is generated by crossing inbred PHAJ0 and inbred PHRE 1. The PHBF0 hybrid is generated by crossing inbred PHBF0 and inbred PHRE 1. The PHAJ0 hybrid shows a lower yield, similar harvest moisture and a higher test weight than the PHBF0 hybrid. The PHAJ0 hybrid demonstrates a superior seedling vigor and an earlier GDU to shed and GDU to silk over the PHBF0 hybrid.

The specific combining ability results in Table 4E compare a PHAJ0 hybrid to a PHAP8 hybrid. The PHAJ0 hybrid is produced by crossing inbred PHAJ0 and inbred PHRE1. The PHAP8 hybrid is produced by crossing inbred PHAP8 and inbred PHRE1. The PHAJ0 hybrid has a lower yield than the PHAP8 hybrid, however, the PHAJ0 hybrid has a lower harvest moisture than the PHAP8 hybrid. The GDU to shed and GDU to silk values are lower for the PHAJ0 hybrid.

The specific combining ability results in Table 4F compare a PHAJ0 hybrid to a PHKF6 hybrid. The PHAJ0 hybrid is generated by crossing inbred PHAJ0 and inbred PHRE1. The PHKF6 hybrid is generated by crossing inbred PHKF6 and inbred PHRE1. The PHAJ0 hybrid shows a lower yield than the PHKF6 hybrid, but the PHAJ0 hybrid also demonstrates a lower harvest moisture than the PHKF6 hybrid.

Table 4G results compare the specific combining abilities of inbred PHAJ0 and inbred PHVA0 in hybrid combination with inbred PHRE1. The PHAJ0 hybrid demonstrates a lower yield and a lower harvest moisture, although the differences are not statistically significant.

Table 4H results compare the specific combining abilities of inbred PHAJ0 and inbred PHNP8 in hybrid combination with inbred PHRE1. The PHAJ0 hybrid demonstrates a slightly lower yield, a lower harvest moisture, and a higher test weight than the PHNP8 hybrid.

The results in Table 41 compare the specific combining abilities of inbred PHAJ0 and inbred PH16F in hybrid combination with inbred PHRE1. The PHAJ0 hybrid shows a lower yield, similar harvest moisture and a higher test weight when compared to the PH16F hybrid. In addition, the PHAJ0 hybrid demonstrates a higher seedling vigor and a lower GDU to shed and GDU to silk than the PH16f hybrid.

In Tables 4A through 4I, PHAJ0 has consistently shown the ability to confer good roots, strong stalks, and good dry down in hybrid combination. These traits are best monitored through the data collected for root lodging (RT LDG), stalk lodging (STK LDG), brittle stalks (BRT STK), harvest moisture (MST) and test weight (TST WT).

                                      TABLE 2A                                     __________________________________________________________________________     PAIRED INBRED COMPARISON DATA                                                  VARIETY #1 = PHAJ0                                                             VARIETY #2 = PHK05                                                                         BU  BU      TST SDG EST TIL GDU GDU                                        VAR ACR ACR MST WT  VGR CNT LER SHD SLK                                        #   ABS % MN                                                                               ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   47.1                                                                               77  14.8                                                                               62.0                                                                               5.5 35.3                                                                               2.6 101.4                                                                              103.8                                      2   38.3                                                                               63  15.3                                                                               60.5                                                                               5.9 31.5                                                                               5.0 97.9                                                                               100.1                                      LOCS                                                                               2   2   2   2   21  40  30  64  63                                         REPS                                                                               4   4   4   4   25  66  41  80  79                                         DIFF                                                                               8.8 14  0.4 1.5 0.4 3.8 2.4 3.1 3.6                                        PROB                                                                               .012+                                                                              .010+                                                                              .406                                                                               .351                                                                               .341                                                                               .020+                                                                              .292                                                                               .000#                                                                              .000#                              __________________________________________________________________________                 POL  POL POL  TAS TAS  PLT EAR  RT                                         VAR WT   WT  SC   BLS SZ   HT  HT   LDG                                        #   ABS  % MN                                                                               ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   125.0                                                                               96  5.4  9.0 5.6  69.2                                                                               26.9 85.1                                       2   153.7                                                                               121 6.0  7.8 5.7  68.7                                                                               24.7 45.6                                       LOCS                                                                               12   12  21   4   19   26  26   4                                          REPS                                                                               23   23  27   6   20   29  29   4                                          DIFF                                                                               28.7 25  0.7  1.3 0.1  0.5 2.2  39.5                                       PROB                                                                               .061*                                                                               .031+                                                                              .045+                                                                               .391                                                                               .786 .627                                                                               .024+                                                                               .196                               __________________________________________________________________________                 STA  STK BRT  GRN SCT  EAR TEX  EAR                                        VAR GRN  LDG STK  APP GRN  SZ  EAR  MLD                                        #   ABS  ABS ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   2.5  95.3                                                                               95.5 5.5 5.7  4.0 8.5  6.3                                        2   3.2  94.6                                                                               100.0                                                                               4.8 7.2  4.0 7.5  7.4                                        LOCS                                                                               5    2   2    2   6    1   2    7                                          REPS                                                                               7    4   2    4   7    1   2    9                                          DIFF                                                                               0.7  0.7 4.5  0.8 1.5  0.0 1.0  1.1                                        PROB                                                                               .548 .461                                                                               .500 .500                                                                               .060*    .000#                                                                               .047+                              __________________________________________________________________________                    BAR  DRP   NLF  GOS   HD   FUS                                           VAR   PLT  EAR   BLT  WLT   SMT  ERS                                           #     ABS  ABS   ABS  ABS   ABS  ABS                                  __________________________________________________________________________     TOTAL SUM                                                                               1     91.3 99.4  3.4  6.0   95.9 3.5                                           2     98.2 99.6  5.1  5.5   87.5 3.0                                           LOCS  9    2     3    1     4    1                                             REPS  11   3     6    2     12   2                                             DIFF  6.9  0.2   1.7  0.5   8.4  0.5                                           PROB  .215 .888  .058*      .387                                      __________________________________________________________________________                    GIB  EYE   HC   ECB   ECB  ECB                                           VAR   ERS  SPT   BLT  DPE   1LF  2SC                                           #     ABS  ABS   ABS  ABS   ABS  ABS                                  __________________________________________________________________________     TOTAL SUM                                                                               1     5.8  3.0   4.7  93.8  1.5  3.0                                           2     5.5  5.5   7.3  75.0  6.2  3.3                                           LOCS  2    1     1    1     3    3                                             REPS  4    2     3    2     6    6                                             DIFF  0.3  2.5   2.7  18.8  4.7  0.3                                           PROB  .500                  .020+                                                                               .529                                 __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 2B                                     __________________________________________________________________________     PAIRED INBRED COMPARISON DATA                                                  VARIETY #1 = PHAJ0                                                             VARIETY #2 = PHJ63                                                                         BU  BU      TST SDG EST TIL                                                VAR ACR ACR MST WT  VGR CNT LER                                                #   ABS % MN                                                                               ABS ABS ABS ABS ABS                                        __________________________________________________________________________     TOTAL SUM                                                                              1   47.1                                                                               77  14.8                                                                               62.0                                                                               6.0 36.2                                                                               18.5                                               2   65.4                                                                               108 15.6                                                                               62.0                                                                               6.4 38.4                                                                               0.0                                                LOCS                                                                               2   2   2   2   5   5   2                                                  REPS                                                                               4   4   4   4   8   8   4                                                  DIFF                                                                               18.3                                                                               31  0.8 0.1 0.4 2.2 18.5                                               PROB                                                                               .262                                                                               .271                                                                               .602                                                                               .910                                                                               .541                                                                               .071*                                                                              .210                                       __________________________________________________________________________                  GDU GDU  POL TAS  PLT EAR                                                 VAR  SHD SLK  SC  SZ   HT  HT                                                  #    ABS ABS  ABS ABS  ABS ABS                                         __________________________________________________________________________     TOTAL SUM                                                                              1    96.6                                                                               99.0 4.5 5.5  72.9                                                                               30.8                                                2    103.6                                                                              106.6                                                                               6.5 6.5  71.7                                                                               26.2                                                LOCS 8   8    2   2    5   5                                                   REPS 10  10   2   2    7   7                                                   DIFF 6.9 7.6  2.0 1.0  1.2 4.5                                                 PROB .000#                                                                              .001#                                                                               .000#                                                                              .000#                                                                               .619                                                                               .040+                                       __________________________________________________________________________                  STA STK  GRN EAR  BAR DRP                                                 VAR  GRN LDG  APP MLD  PLT EAR                                                 #    ABS ABS  ABS ABS  ABS ABS                                         __________________________________________________________________________     TOTAL SUM                                                                              1    3.3 95.3 5.5 7.0  96.5                                                                               99.4                                                2    1.8 92.2 3.8 6.0  95.7                                                                               100.0                                               LOCS 2   2    2   1    2   2                                                   REPS 4   4    4   1    4   3                                                   DIFF 1.5 3.1  1.8 1.0  0.8 0.6                                                 PROB .205                                                                               .489 .090*    .260                                                                               .500                                        __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 2C                                     __________________________________________________________________________     PAIRED INBRED COMPARISON DATA                                                  VARIETY #1 = PHAJ0                                                             VARIETY #2 = PHW06                                                                         BU  BU      TST SDG EST TIL GDU GDU                                        VAR ACR ACR MST WT  VGR CNT LER SHD SLK                                        #   ABS % MN                                                                               ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   47.1                                                                               77  14.8                                                                               62.0                                                                               5.5 37.5                                                                               2.5 102.7                                                                              105.3                                      2   53.6                                                                               88  13.8                                                                               62.1                                                                               5.0 35.3                                                                               5.9 112.1                                                                              116.1                                      LOCS                                                                               2   2   2   2   14  32  29  61  60                                         REPS                                                                               4   4   4   4   18  42  38  85  84                                         DIFF                                                                               6.5 11  1.0 0.2 0.5 2.2 3.4 9.5 10.8                                       PROB                                                                               .290                                                                               .294                                                                               .467                                                                               .925                                                                               .468                                                                               .163                                                                               .213                                                                               .000#                                                                              .000#                              __________________________________________________________________________                 POL  POL POL  TAS TAS  PLT EAR  RT                                         VAR WT   WT  SC   BLS SZ   HT  HT   LDG                                        #   ABS  % MN                                                                               ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   117.1                                                                               103 5.3  9.0 5.6  67.5                                                                               26.4 82.2                                       2   96.0 84  4.6  8.0 4.2  69.1                                                                               26.0 98.6                                       LOCS                                                                               31   31  20   4   19   22  21   3                                          REPS                                                                               51   51  24   6   20   25  24   3                                          DIFF                                                                               21.0 20  0.8  1.0 1.4  1.6 0.4  16.3                                       PROB                                                                               .005#                                                                               .003#                                                                              .063*                                                                               .391                                                                               .000#                                                                               .409                                                                               .666 .434                               __________________________________________________________________________                 STA  STK BRT  GRN SCT  EAR BAR  DRP                                        VAR GRN  LDG STK  APP GRN  MLD PLT  EAR                                        #   ABS  ABS ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   2.5  96.9                                                                               95.5 5.5 5.5  6.9 91.3 98.9                                       2   2.8  92.2                                                                               100.0                                                                               5.5 5.3  7.0 99.0 100.0                                      LOCS                                                                               5    3   2    2   6    7   9    1                                          REPS                                                                               7    5   2    4   7    8   11   2                                          DIFF                                                                               0.3  4.6 4.5  0.0 0.2  0.1 7.7  1.1                                        PROB                                                                               .529 .478                                                                               .500 .000#                                                                              .695 .788                                                                               .157                                    __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 2D                                     __________________________________________________________________________     PAIRED INBRED COMPARISON DATA                                                  VARIETY #1 = PHAJ0                                                             VARIETY #2 = PHJ40                                                                         BU  BU      TST SDG EST TIL GDU GDU                                        VAR ACR ACR MST WT  VGR CNT LER SHD SLK                                        #   ABS % MN                                                                               ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   47.1                                                                               77  14.8                                                                               62.0                                                                               5.5 36.3                                                                               3.0 101.4                                                                              103.9                                      2   68.1                                                                               112 15.3                                                                               62.3                                                                               5.4 35.7                                                                               1.0 111.3                                                                              113.1                                      LOCS                                                                               2   2   2   2   21  42  33  75  74                                         REPS                                                                               4   4   4   4   25  71  47  106 105                                        DIFF                                                                               21.0                                                                               35  0.5 0.3 0.1 0.6 2.0 9.9 9.2                                        PROB                                                                               .153                                                                               .166                                                                               .406                                                                               .302                                                                               .715                                                                               .473                                                                               .112                                                                               .000#                                                                              .000#                              __________________________________________________________________________                 POL POL POL TAS TAS PLT EAR RT  STA                                        VAR WT  WT  SC  BLS SZ  HT  HT  LDG GRN                                        #   ABS % MN                                                                               ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   113.2                                                                              99  5.3 9.0 5.6 68.8                                                                               27.1                                                                               85.1                                                                               2.5                                        2   103.4                                                                              91  5.1 8.8 4.6 66.0                                                                               28.8                                                                               93.8                                                                               4.7                                        LOCS                                                                               34  34  22  5   20  26  26  4   5                                          REPS                                                                               61  61  29  8   21  29  29  4   7                                          DIFF                                                                               9.7 8   0.1 0.2 1.0 2.8 1.7 8.6 2.2                                        PROB                                                                               .122                                                                               .157                                                                               .692                                                                               .374                                                                               .002#                                                                              .031+                                                                              .100                                                                               .596                                                                               .097*                              __________________________________________________________________________                 STK BRT GRN SCT TEX EAR BAR DRP NLF                                        VAR LDG STK APP GRN EAR MLD PLT EAR BLT                                        #   ABS ABS ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   96.9                                                                               95.5                                                                               5.5 5.6 8.0 6.9 92.2                                                                               99.4                                                                               4.0                                        2   97.7                                                                               100.0                                                                              7.4 6.9 7.0 6.9 99.4                                                                               99.4                                                                               8.0                                        LOCS                                                                               3   2   2   7   1   7   10  2   1                                          REPS                                                                               5   2   4   8   1   8   12  3   1                                          DIFF                                                                               0.8 4.5 1.9 1.3 1.0 0.0 7.2 0.0 4.0                                        PROB                                                                               .734                                                                               .500                                                                               .245                                                                               .022+   .000#                                                                              .135                                                                               .979                                   __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 3A                                     __________________________________________________________________________     Average Inbred By Tester Performance Comparing PHK05 To PHAJ0                  Crossed To The Same Inbred Testers And Grown In The Same Experiments.          All Values Are Expressed As Percent Of The Experiment Mean                     Except Predicted RM, Selection Index, and Yield (BU/ACR)                                        SEL BU      PRM    TST                                               INBRED                                                                               PRM IND ACR YLD SHD MST                                                                               WT                                         __________________________________________________________________________     TOTAL  REPLIC.                                                                              40  44  44  44  5   45 38                                         MEAN WTS                                                                              PHK05 76  99  117 99  81  95 54                                         MEAN WTS                                                                              PHAJ0 79  96  115 97  82  98 54                                                DIFF  3   3   2   2   1   3  0                                          __________________________________________________________________________                  SDG EST GDU GDU STK PLT                                                                               EAR                                               INBRED                                                                               VGR CNT SHD SLK CNT HT HT                                         __________________________________________________________________________     TOTAL  REPLIC.                                                                              29  32  8   2   44  24 24                                         MEAN WTS                                                                              PHK05 110 103 98  98  100 102                                                                               103                                        MEAN WTS                                                                              PHAJ0 96  98  100 98  100 98 100                                               DIFF  14  6   1   0   0   4  3                                          __________________________________________________________________________                   RT   STA  STK  BRT  GRN                                                 INBRED LDG  GRN  LDG  STK  APP                                          __________________________________________________________________________     TOTAL  REPLIC.                                                                               10   26   44   9    15                                           MEAN WTS                                                                              PHK05  81   100  100  100  100                                          MEAN WTS                                                                              PHAJ0  97   109  99   100  101                                                 DIFF   16   9    1         1                                            __________________________________________________________________________

                                      TABLE 4A                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PH54B/PHRE1                                                                              BU   BU        TST  SDG                                         VAR       PRM  ACR  ACR  MST  WT   VGR                                         #    PRM  SHD  ABS  % MN % MN ABS  % MN                                __________________________________________________________________________     TOTAL SUM                                                                              1    81   83   117.2                                                                               98   95   54.9 105                                         2    80   87   126.7                                                                               106  95   53.0 94                                          LOCS 10   11   54   54   55   33   57                                          REPS 10   11   76   76   77   44   94                                          DIFF 0    4    9.5  8    0    2.0  10                                          PROB .747 .000#                                                                               .000#                                                                               .000#                                                                               .978 .000#                                                                               .003#                               __________________________________________________________________________                    EST   GDU  GDU   STK  PLT   EAR                                           VAR  CNT   SHD  SLK   CNT  HT    HT                                            #    % MN  % MN % MN  % MN % MN  % MN                                __________________________________________________________________________     TOTAL SUM 1    101   98   97    100  98    102                                           2    99    102  101   99   102   108                                           LOCS 50    21   14    75   36    36                                            REPS 85    33   22    108  53    53                                            DIFF 3     4    4     0    4     6                                             PROB .020+ .000#                                                                               .000# .327 .000# .000#                               __________________________________________________________________________                    RT    STA  STK   BRT  GRN   DRP                                           VAR  LDG   GRN  LDG   STK  APP   EAR                                           #    % MN  % MN % MN  % MN % MN  % MN                                __________________________________________________________________________     TOTAL SUM 1    98    112  100   100  102   100                                           2    93    85   104   100  98    100                                           LOCS 28    28   47    10   19    33                                            REPS 42    38   66    10   23    40                                            DIFF 5     27   4     1    4     1                                             PROB .115  .000#                                                                               .001# .193 .308  .022+                               __________________________________________________________________________                 NLF GOS HD  FUS GIB EYE ECB ECB ECB                                        VAR BLT WLT SMT ERS ERS SPT 1LF 2SC 2IT                                        #   ABS ABS ABS ABS ABS ABS ABS ABS ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   6.3 5.0 100.0                                                                              3.5 6.9 5.0 2.5 5.2 4.3                                        2   5.3 5.5 99.2                                                                               3.5 6.5 4.5 1.8 5.3 5.9                                        LOCS                                                                               2   1   3   1   4   1   3   3   1                                          REPS                                                                               5   2   6   2   8   2   8   7   3                                          DIFF                                                                               1.1 0.5 0.8 0.0 0.4 0.5 0.7 0.1 1.7                                        PROB                                                                               .314    .423    .391    .184                                                                               .926                                   __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4B                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PH90A/PHRE1                                                                           BU  BU      TST SDG EST GDU                                        VAR     PRM ACR ACR MST WT  VGR CNT SHD                                        #   PRM SHD ABS % MN                                                                               % MN                                                                               ABS % MN                                                                               % MN                                                                               % MN                               __________________________________________________________________________     TOTAL SUM                                                                              1   81  83  115.2                                                                              98  95  54.9                                                                               105 101 98                                         2   83  87  116.8                                                                              99  100 54.1                                                                               95  98  102                                        LOCS                                                                               10  12  52  52  53  33  52  45  18                                         REPS                                                                               10  12  67  67  68  42  84  70  27                                         DIFF                                                                               2   4   1.5 1   4   0.8 11  3   4                                          PROB                                                                               .020+                                                                              .001#                                                                              .454                                                                               .607                                                                               .000#                                                                              .078*                                                                              .012+                                                                              .014+                                                                              .000#                              __________________________________________________________________________                 GDU STK PLT EAR RT  STA STK BRT GRN                                        VAR SLK CNT HT  HT  LDG GRN LDG STK APP                                        #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                               __________________________________________________________________________     TOTAL SUM                                                                              1   96  100 98  102 99  112 100 100 102                                        2   102 100 94  94  107 89  100 100 100                                        LOCS                                                                               10  63  31  31  20  25  45  10  19                                         REPS                                                                               16  83  40  40  28  34  57  10  21                                         DIFF                                                                               5   0   3   7   8   22  0   0   2                                          PROB                                                                               .000#                                                                              .606                                                                               .000#                                                                              .000#                                                                              .025+                                                                              .009#                                                                              .971                                                                               .343                                                                               725                                __________________________________________________________________________                 DRP  NLF GOS  HD  FUS  GIB EYE  ECB                                        VAR EAR  BLT WLT  SMT ERS  ERS SPT  2SC                                        #   % MN ABS ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   100  6.3 5.0  100.0                                                                              3.5  6.9 5.0  7.0                                        2   100  5.5 5.5  100.0                                                                              2.5  6.5 5.0  7.0                                        LOCS                                                                               33   2   1    3   1    4   1    1                                          REPS                                                                               35   5   2    6   2    7   2    1                                          DIFF                                                                               0    0.8 0.5  0.0 1.0  0.4 0.0  0.0                                        PROB                                                                               .352 .500     1.00     .391                                        __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4C                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHKM5/PHRE1                                                                           BU  BU      TST SDG EST GDU                                        VAR     PRM ACR ACR MST WT  VGR CNT SHD                                        #   PRM SHD ABS % MN                                                                               % MN                                                                               ABS % MN                                                                               % MN                                                                               % MN                               __________________________________________________________________________     TOTAL SUM                                                                              1   80  82  122.6                                                                              103 94  54.9                                                                               109 102 99                                         2   82  85  129.3                                                                              107 98  54.5                                                                               108 99  101                                        LOCS                                                                               7   8   35  35  35  19  40  36  17                                         REPS                                                                               7   8   53  53  53  30  71  69  29                                         DIFF                                                                               2   3   6.7 5   5   0.4 1   3   3                                          PROB                                                                               .011+                                                                              .000#                                                                              .044+                                                                              .086*                                                                              .000#                                                                              .386                                                                               .793                                                                               .159                                                                               .000#                              __________________________________________________________________________                 GDU  STK PLT  EAR RT   STA STK  BRT                                        VAR SLK  CNT HT   HT  LDG  GRN LDG  STK                                        #   % MN % MN                                                                               % MN % MN                                                                               % MN % MN                                                                               % MN % MN                               __________________________________________________________________________     TOTAL SUM                                                                              1   97   100 99   102 97   117 101  101                                        2   100  100 98   101 107  105 107  98                                         LOCS                                                                               9    51  24   24  20   18  29   4                                          REPS                                                                               15   80  40   40  33   27  47   4                                          DIFF                                                                               3    0   1    1   9    12  7    3                                          PROB                                                                               .002#                                                                               .475                                                                               .353 .512                                                                               .039+                                                                               .245                                                                               .000#                                                                               .391                               __________________________________________________________________________                 GRN  DRP NLF  GOS HD   FUS GIB  EYE                                        VAR APP  EAR BLT  WLT SMT  ERS ERS  SPT                                        #   % MN % MN                                                                               ABS  ABS ABS  ABS ABS  ABS                                __________________________________________________________________________     TOTAL SUM                                                                              1   102  100 6.3  5.0 100.0                                                                               3.5 6.9  5.0                                        2   95   100 6.3  4.5 92.4 3.5 6.0  4.5                                        LOCS                                                                               9    16  2    1   2    1   4    1                                          REPS                                                                               13   23  5    2   4    2   8    2                                          DIFF                                                                               6    0   0.0  0.5 7.6  0.0 0.9  0.5                                        PROB                                                                               .192 .807                                                                               .000#    .058*    .235                                    __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4D                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHBF0/PHRE1                                                                           BU  BU      TST SDG EST                                            VAR     PRM ACR ACR MST WT  VGR CNT                                            #   PRM SHD ABS % MN                                                                               % MN                                                                               ABS % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   85  82  125.9                                                                              89  94  62.9                                                                               125 107                                            2   84  94  152.0                                                                              108 93  59.1                                                                               103 103                                            LOCS                                                                               1   3   3   3   4   4   10  10                                             REPS                                                                               1   3   4   4   5   4   26  25                                             DIFF                                                                               1   13  26.1                                                                               19  1   3.8 22  4                                              PROB    .005#                                                                              .045+                                                                              .043+                                                                              .570                                                                               .021+                                                                              .006#                                                                              .209                                   __________________________________________________________________________                 GDU GDU STK PLT EAR RT  STA STK                                            VAR SHD SLK CNT HT  HT  LDG GRN LDG                                            #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   95  95  98  95  96  99  89  97                                             2   105 104 100 100 107 97  92  103                                            LOCS                                                                               4   4   7   4   4   3   4   3                                              REPS                                                                               9   9   12  6   6   4   6   4                                              DIFF                                                                               10  8   2   5   11  3   4   5                                              PROB                                                                               .004#                                                                              .003#                                                                              .081*                                                                              .051*                                                                              .047+                                                                              .565                                                                               .774                                                                               .496                                   __________________________________________________________________________                 BRT DRP NLF GOS HD  FUS GIB EYE                                            VAR STK EAR BLT WLT SMT ERS ERS SPT                                            #   % MN                                                                               % MN                                                                               ABS ABS ABS ABS ABS ABS                                    __________________________________________________________________________     TOTAL SUM                                                                              1   101 99  6.3 5.0 100.0                                                                              3.0 6.8 5.0                                            2   101 100 5.8 4.5 100.0                                                                              4.0 6.5 4.0                                            LOCS                                                                               1   2   2   1   2   1   4   1                                              REPS                                                                               1   2   5   2   4   1   7   2                                              DIFF                                                                               0   1   0.5 0.5 0.0 1.0 0.3 1.0                                            PROB    .500                                                                               .500    1.00    .638                                       __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4E                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHAP8/PHRE1                                                                            BU   BU       TST                                                 VAR      PRM ACR  ACR MST  WT                                                  #   PRM  SHD ABS  % MN                                                                               % MN ABS                                         __________________________________________________________________________     TOTAL SUM                                                                              1   85   82  125.9                                                                               89  94   62.7                                                2   94   92  152.4                                                                               109 107  63.0                                                LOCS                                                                               1    3   3    3   4    3                                                   REPS                                                                               1    3   4    4   5    3                                                   DIFF                                                                               9    10  26.5 20  13   0.3                                                 PROB     .003#                                                                              .157 .160                                                                               .107 .862                                        __________________________________________________________________________                 SDG  EST GDU  GDU STK  PLT                                                 VAR VGR  CNT SHD  SLK CNT  HT                                                  #   % MN % MN                                                                               % MN % MN                                                                               % MN % MN                                        __________________________________________________________________________     TOTAL SUM                                                                              1   129  107 94   95  99   94                                                  2   124  105 103  102 100  99                                                  LOCS                                                                               9    10  3    3   4    3                                                   REPS                                                                               24   25  7    7   6    4                                                   DIFF                                                                               4    3   8    8   1    6                                                   PROB                                                                               .690 .482                                                                               .000#                                                                               .001#                                                                              .391 .201                                        __________________________________________________________________________                 EAR  RT  STA  STK BRT  DRP                                                 VAR HT   LDG GRN  LDG STK  EAR                                                 #   % MN % MN                                                                               % MN % MN                                                                               % MN % MN                                        __________________________________________________________________________     TOTAL SUM                                                                              1   98   99  85   97  101  99                                                  2   100  92  121  99  99   99                                                  LOCS                                                                               3    3   3    3   1    2                                                   REPS                                                                               4    4   4    4   1    2                                                   DIFF                                                                               2    8   36   2   2    0                                                   PROB                                                                               .777 .581                                                                               .309 .831     1.00                                        __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4F                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHKF6/PHRE1                                                                       BU  BU      SDG GDU GDU                                                VAR     ACR ACR MST VGR SHD SLK                                                #   PRM ABS % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                       __________________________________________________________________________     TOTAL SUM                                                                              1   78  154.4                                                                              94  94  98  100 99                                                 2   84  173.8                                                                              105 104 115 105 108                                                LOCS                                                                               1   4   4   4   8   3   2                                                  REPS                                                                               1   4   4   4   9   3   2                                                  DIFF                                                                               5   19.4                                                                               11  10  16  4   9                                                  PROB    .060*                                                                              .050*                                                                              .001#                                                                              .206                                                                               .229                                                                               .297                                       __________________________________________________________________________                 STK PLT EAR RT  STA STK DRP                                                VAR CNT HT  HT  LDG GRN LDG EAR                                                #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                       __________________________________________________________________________     TOTAL SUM                                                                              1   100 98  99  98  115 100 100                                                2   100 102 110 116 119 98  100                                                LOCS                                                                               13  5   5   5   3   1   1                                                  REPS                                                                               17  8   8   8   4   3   3                                                  DIFF                                                                               0   5   11  19  4   2   0                                                  PROB                                                                               .732                                                                               .080*                                                                              .005#                                                                              .388                                                                               .721                                               __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4G                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHVA0/PHRE1                                                                       BU  BU      SDG GDU GDU                                                VAR     ACR ACR MST VGR SHD SLK                                                #   PRM ABS % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                       __________________________________________________________________________     TOTAL SUM                                                                              1   78  145.8                                                                              98  90  97  100 101                                                2   80  153.3                                                                              101 96  79  103 101                                                LOCS                                                                               2   5   5   5   7   3   1                                                  REPS                                                                               2   5   5   5   8   3   1                                                  DIFF                                                                               2   7.5 4   6   18  2   0                                                  PROB                                                                               .171                                                                               .246                                                                               .370                                                                               .149                                                                               .104                                                                               .423                                           __________________________________________________________________________                 STK PLT EAR RT  STA STK GRN                                                VAR CNT HT  HT  LDG GRN LDG APP                                                #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                       __________________________________________________________________________     TOTAL SUM                                                                              1   99  98  95  103 99  88  119                                                2   94  97  96  102 109 101 89                                                 LOCS                                                                               12  3   3   3   1   1   1                                                  REPS                                                                               17  4   4   4   2   1   1                                                  DIFF                                                                               4   0   1   1   10  13  30                                                 PROB                                                                               .215                                                                               .423                                                                               .815                                                                               .184                                                   __________________________________________________________________________                 NLF GOS HD  FUS GIB EYE                                                    VAR BLT WLT SMT ERS ERS SPT                                                    #   ABS ABS ABS ABS ABS ABS                                            __________________________________________________________________________     TOTAL SUM                                                                              1   6.0 5.0 100.0                                                                              3.5 6.8 5.0                                                    2   4.0 6.0 90.9                                                                               5.0 7.3 2.5                                                    LOCS                                                                               2   1   2   1   3   1                                                      REPS                                                                               3   2   4   2   6   2                                                      DIFF                                                                               2.0 1.0 9.1 1.5 0.5 2.5                                                    PROB                                                                               .000#   .500    .423                                               __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4H                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PHNP8/PHRE1                                                                           BU  BU      TST SDG EST                                            VAR     PRM ACR ACR MST WT  VGR CNT                                            #   PRM SHD ABS % MN                                                                               % MN                                                                               ABS % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   82  81  86.6                                                                               100 89  48.5                                                                               91  95                                             2   84  89  90.0                                                                               108 97  43.8                                                                               110 105                                            LOCS                                                                               1   1   3   3   3   3   1   3                                              REPS                                                                               1   1   3   3   3   3   1   3                                              DIFF                                                                               2   7   3.4 8   8   4.7 18  10                                             PROB        .775                                                                               .655                                                                               .043+                                                                              .007#   .200                                   __________________________________________________________________________                 GDU STK PLT EAR STA STK GRN DRP                                            VAR SHD CNT HT  HT  GRN LDG APP EAR                                            #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   97  100 109 116 98  100 111 100                                            2   105 100 108 102 112 98  93  100                                            LOCS                                                                               1   5   2   2   1   3   2   2                                              REPS                                                                               1   5   2   2   1   3   2   2                                              DIFF                                                                               8   0   1   14  14  2   18  0                                              PROB    1.00                                                                               .500                                                                               .033+   .619                                                                               .121                                                                               1.00                                   __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

                                      TABLE 4I                                     __________________________________________________________________________     VARIETY #1 = PHAJ0/PHRE1                                                       VARIETY #2 = PH16F/PHRE1                                                                           BU  BU      TST SDG EST                                            VAR     PRM ACR ACR MST WT  VGR CNT                                            #   PRM SHD ABS % MN                                                                               % MN                                                                               ABS % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   85  82  125.9                                                                              89  94  62.9                                                                               125 107                                            2   84  89  143.6                                                                              102 93  58.9                                                                               109 103                                            LOCS                                                                               1   3   3   3   4   4   10  10                                             REPS                                                                               1   3   4   4   5   4   26  25                                             DIFF                                                                               1   7   17.7                                                                               13  1   4.0 16  5                                              PROB    .005#                                                                              .074*                                                                              .103                                                                               .802                                                                               .002#                                                                              .081*                                                                              .286                                   __________________________________________________________________________                 GDU GDU STK PLT EAR RT  STA STK                                            VAR SHD SLK CNT HT  HT  LDG GRN LDG                                            #   % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                                                               % MN                                   __________________________________________________________________________     TOTAL SUM                                                                              1   95  95  98  94  98  99  85  97                                             2   101 100 99  98  96  98  70  103                                            LOCS                                                                               4   4   8   3   3   3   3   3                                              REPS                                                                               9   9   14  4   4   4   4   4                                              DIFF                                                                               6   5   1   4   1   2   15  5                                              PROB                                                                               .003#                                                                              .016+                                                                              .413                                                                               .155                                                                               .721                                                                               .695                                                                               .615                                                                               .404                                   __________________________________________________________________________                 BRT DRP NLF GOS HD  FUS GIB EYE                                            VAR STK EAR BLT WLT SMT ERS ERS SPT                                            #   % MN                                                                               % MN                                                                               ABS ABS ABS ABS ABS ABS                                    __________________________________________________________________________     TOTAL SUM                                                                              1   101 99  6.3 5.0 100.0                                                                              3.5 6.9 5.0                                            2   101 100 6.9 7.0 96.2                                                                               3.5 5.8 5.5                                            LOCS                                                                               1   2   2   1   2   1   4   1                                              REPS                                                                               1   2   5   2   4   2   8   2                                              DIFF                                                                               0   1   0.6 2.0 3.8 0.0 1.1 0.5                                            PROB    .500                                                                               .639    .258    .266                                       __________________________________________________________________________      * = 10% SIG                                                                    + = 5% SIG                                                                     # = 1% SIG                                                               

Deposits

Applicants have made a deposit of at least 2500 seeds of Inbred Maize Line PHAJ0 with the American Type Culture Collection (ATCC), Rockville, Md. 20852 U.S.A., ATCC Deposit No. 97574. The seeds deposited with the ATCC on May 20, 1996 were taken from the same deposit maintained by Pioneer Hi-Bred International, Inc., 700 Capital Square, 400 Locust Street, Des Moines, Iowa 50309-2340 since prior to the filing date of this application. This deposit of the Inbred Maize Line PHAJ0 will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant has satisfied all the requirements of 37 C.F.R. §§1.801-1.809, including providing an indication of the viability of the sample. Applicant imposes no restrictions on the availability of the deposited material from the ATCC; however, Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of its rights granted under this patent.

The foregoing invention has been described in detail by way of illustration and example for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications such as single gene modifications and mutations, somoclonal variants, variant individuals selected from large populations of the plants of the instant inbred and the like may be practiced within the scope of the invention, as limited only by the scope of the appended claims. 

What is claimed is:
 1. Seed of maize inbred line designated PHAJ0 and having ATCC Accession No.
 97574. 2. A maize plant produced by the seed of claim 1 and its plant parts.
 3. Pollen of the plant of claim
 2. 4. An ovule of the plant of claim
 2. 5. A maize plant having all the physiological and morphological characteristics of a maize plant produced by seed of maize inbred line designated PHAJ0 having ATCC Accession No.
 97574. 6. The maize plant of claim 5, wherein the maize plant is further characterized as being male sterile.
 7. A tissue culture of regenerable cells of an inbred maize plant PHAJ0, wherein the tissue culture regenerates maize plants capable of expressing all the morphological and physiological characteristics of inbred maize plant PHAJ0, seed of said inbred maize plant PHAJ0 having ATCC Accession No.
 97574. 8. The tissue culture of claim 7, the tissue culture selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks, stalks, cells and protoplasts thereof.
 9. A maize plant regenerated from the tissue culture of claim 7, the regenerated maize plant being capable of expressing all the morphological and physiological characteristics of inbred maize plant PHAJ0, seed of said inbred maize plant PHAJ0 having ATCC Accession No.
 97574. 10. A method for producing first generation (F₁) hybrid maize seed comprising crossing a first inbred parent maize plant with a second inbred parent maize plant and harvesting the resultant first generation (F₁) hybrid maize seed, wherein said first or second parent maize plant is the maize plant of claim
 2. 11. The method of claim 10 wherein the maize plant of claim 2 serves as the female parent in the cross.
 12. The method of claim 10 wherein the maize plant of claim 2 serves as the male parent in the cross.
 13. An F₁ hybrid seed and plant grown therefrom produced by crossing an inbred maize plant according to claim 2 with another, different maize plant. 